本期文章：《德国应用化学》：Online/在线发表

中山大学张元庆团队开发了一种用于COVID-19血清评估的检测范围可调的双编码微球免疫分析法。相关研究成果发表在2022年7月15日出版的《德国应用化学》。

2019冠状病毒病（COVID-19）患者（包括无症状患者）的血清学检测可以为疾病进展和预后提供信息。多重性、高灵敏度和更宽检测范围的检测方法将有助于识别和治疗COVID-19。

该文中，研究人员将颜色-尺寸双编码微球和滚环扩增(RCA)集成到微球荧光免疫分析中，并在尺寸分选芯片中实现，以实现高通量和灵敏的检测。研究人员使用该分析来定量针对spike S1、核衣壳、受体结合域抗原的COVID-19抗体，并检测炎症生物标记物，包括白细胞介素-6、白细胞介素-1β、降钙素原、C-反应蛋白，其浓度范围从pg mL^-1到μg mL^-1，因为与RCA整合的不同大小的微球导致检测范围可调。

该方法可以很容易地修改，以同时测量更多不同数量级的COVID-19血清分子。

附：英文原文

Title: A Dual-Encoded Bead-Based Immunoassay with Tunable Detection Range for COVID-19 Serum Evaluation

Author: Zhun Lin, Jie Zhang, Zhengyu Zou, Gen Lu, Minhao Wu, Li Niu, Yuanqing Zhang

Issue&Volume: 2022-07-15

Abstract: Serological assay for coronavirus disease 2019 (COVID-19) patients including asymptomatic cases can inform disease progression and prognosis. A detection method taking into account multiplex, high sensitivity, and a wider detection range will help to identify and treat COVID-19. Here we integrated color-size dual-encoded beads and rolling circle amplification (RCA) into bead-based fluorescence immunoassay implemented in a size sorting chip to achieve high-throughput and sensitive detection. We used the assay for quantifying COVID-19 antibodies against spike S1, nucleocapsid, the receptor binding domain antigens, and detecting inflammatory biomarkers including interleukin-6, interleukin-1β, procalcitonin, C-reactive protein whose concentrations range from pg mL  -1  to μg mL  -1  as different size beads integrating with RCA result in tunable detection range. The assay can be readily modified to simultaneously measure more COVID-19 serological molecules differing by orders of magnitude.

DOI: 10.1002/anie.202203706